United had about 1,100 employees before the first wave of cuts last year.
About 250 employees were made redundant during the first round of redundancies, saving the club between £8m and £10m.
United chief executive Omar Berrada said earlier this year that between 150 and 200 redundancies were set to follow.
Defeat in Bilbao means United will be without European football next season for only the second time since 1990, leaving a £100m hole in the club’s finances.
Ratcliffe has embarked on a series of cost-cutting initiatives since purchasing a minority stake in the club in February 2024.
Club ambassadors, including Sir Alex Ferguson, have seen their roles removed, while a canteen for staff was closed and replaced with the offer of free fruit.
Ratcliffe and fellow co-owner Avram Glazer attended Wednesday’s final in Bilbao, with Ferguson pictured alongside them.
Sources have told Sport that a number of cars were driven to Bilbao before the final for the use of Ratcliffe and senior club officials during their time in Spain.
The club stuck to plans to hold a barbecue for the first-team squad and family members at Carrington following the defeat by Spurs.
But the latest cutbacks come against a bleak financial backdrop, with United losing more than £370m over the past five years. According to their 2024 accounts, they owe £313m in outstanding transfer payments to other clubs.
