Oldham Athletic will visit MK Dons in their first game back in League Two after three seasons of non-league football.
The Latics became the first of the founding Premier League member clubs to drop out of the English Football League when they were relegated to the National League in 2022.
Oldham won promotion via the play-offs last season, beating Southend United 3-2 at Wembley last month.
Runaway National League champions Barnet host Fleetwood Town at The Hive, while Cambridge United face Cheltenham Town at home following their relegation from League One.
Bristol Rovers will take on Harrogate Town, while Walsall – long-time leaders last season – will look to bounce back strongly from the disappointment of defeat in the play-off final to AFC Wimbledon when they host Swindon Town.
Relegated Shrewsbury Town will face Bromley, while Crawley Town, who were also relegated after just one season in League One, will travel to Grimsby Town.
All League Two matches on the opening day of the season – Saturday, 2 August – will kick off at 15:00 BST.
